Figure 1. Standardization of an electrical resistance-based assay as a measure of SARS-CoV-2 induced CPE and anti-SARS-CoV-2 activity.

VeroE6 cells were seeded into a CytoView-Z 96-well plate and cells were allowed to stabilize overnight, as measured by electrical resistance. A) SARS-CoV-2 was titrated in 10-fold dilutions ranging from 10–0.0001 MOI. Resistance was measured every minute over the course of 72 hours. Solid lines indicate the mean, dotted lines indicate the standard error of three replicates. B) Median time to death calculations based on raw resistance data for each MOI. C) Remdesivir was titrated in 6-fold dilutions ranging from 50–0.006 μM. After infection at an MOI of 0.01, resistance was monitored for 48 h.p.i. and D) percent inhibition was determined at the 48 hour timepoint.