Table 1. Identification of 6 nontryptic and 52 semitryptic NISTmAb peptides with their corresponding sequences, elution times, and m/z (charge).
| Cleavage | Chain | Peptide | Sequence | RT (min) | m/z (charge) |
|---|---|---|---|---|---|
| Nontryptic | Heavy | 28–38 | SLSTAGMSVGW | 37.0 | 1095.5138 (+1) |
| 162–183 | NSGALTSGVHTFPAVLQSSGLY | 47.2 | 1103.5613 (+2) | ||
| 184–201 | SLSSVVTVPSSSLGTQTY | 37.2 | 906.9647 (+2) | ||
| Light | 71–86 | TLTISSLQPDDFATYY | 47.8 | 917.9414 (+2) | |
| 86–95 | YCFQGSGYPF | 36.3 | 1225.4990 (+1) | ||
| 87–95 | CFQGSGYPF | 32.4 | 1062.4354 (+1), 531.7214 (+2) | ||
| Semitryptic | Heavy | 6–18 | ESGPALVKPTQTL | 30.0 | 1340.7430 (+1), 670.8743 (+2) |
| 6–24 | ESGPALVKPTQTLTLTCTF | 44.2 | 1032.5341 (+2), 688.6931 (+3) | ||
| 6–27 | ESGPALVKPTQTLTLTCTFSGF | 49.8 | 1178.0945 (+2), 785.7338 (+3) | ||
| 6–38 | ESGPALVKPTQTLTLTCTFSGFSLSTAGMSVGW | 60.9 | 1716.3461 (+2), 1144.5663 (+3) | ||
| 25–40 | SGFSLSTAGMSVGWIR | 48.2 | 1655.8196 (+1), 828.4121 (+2), 552.6110 (+3) | ||
| 28–40 | SLSTAGMSVGWIR | 39.6 | 1364.6995 (+1), 682.8528 (+2), 455.5711 (+3) | ||
| 39–45 | IRQPPGK | 11.0 | 795.4828 (+1), 398.2450 (+2) | ||
| 46–55 | ALEWLADIWW | 19.6 | 1302.6418 (+1), 651.8240 (+2), 434.8851 (+3) | ||
| 84–95 | VTNMDPADTATY | 24.1 | 1298.5563 (+1), 649.7821 (+2) | ||
| 84–96 | VTNMDPADTATYY | 27.9 | 1461.6192 (+1), 731.3139 (+2) | ||
| 96–99 | YCAR | 9.1 | 569.2501 (+1), 285.1286 (+2) | ||
| 106–124 | YFDVWGQGTTVTVSSASTK | 37.5 | 1017.4943 (+2) | ||
| 151–161 | DYFPEPVTVSW | 47.5 | 1339.6217 (+1), 670.3137 (+2) | ||
| 151–183 | DYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLY | 60.4 | 1763.8600 (+2), 1176.2424 (+3), 882.4336 (+4) | ||
| 151–201 | DYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTY | 66.0 | 1774.2085 (+3), 1330.9107 (+4), 1065.9287 (+5) | ||
| 162–213 | NSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTK | 55.3 | 1798.2434 (+3), 1348.9343 (+4), 1079.3483 (+5) | ||
| 184–213 | SLSSVVTVPSSSLGTQTYICNVNHKPSNTK | 35.5 | 1603.3126 (+2), 1069.2094 (+3), 802.1594 (+4) | ||
| 184–216 | SLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDK | 34.5 | 1774.4087 (+2), 1183.2745 (+3), 887.7074 (+4) | ||
| 202–213 | ICNVNHKPSNTK | 11.1 | 1411.7106 (+1), 706.3584 (+2), 471.2417 (+3), 353.6830 (+4) | ||
| 217–245 | RVEPKSCDKTHTCPPCPAPELLGGPSVFL | 35.5 | 1625.2948 (+2), 1083.8637 (+3) | ||
| 222–244 | SCDKTHTCPPCPAPELLGGPSVF | 43.8 | 1264.0719 (+2), 843.0508 (+3) | ||
| 226–244 | THTCPPCPAPELLGGPSVF | 45.6 | 1018.9829 (+2), 679.6586 (+3) | ||
| 245–251 | LFPPKPK | 21.4 | 826.5180 (+1), 413.7627 (+2), 276.1775 (+3) | ||
| 310–320 | TVLHQDWLNGK | 28.5 | 1310.6865 (+1), 655.8463 (+2), 437.5666 (+3) | ||
| 348–352 | EPQVY | 17.4 | 635.3033 (+1), 318.1553 (+2) | ||
| 353–363 | TLPPSREEMTK | 17.9 | 1288.6549 (+1), 644.8307 (+2) | ||
| 396–408 | TTPPVLDSDGSFF | 44.0 | 1382.6441 (+1), 691.8267 (+2) | ||
| 396–410 | TTPPVLDSDGSFFLY | 51.3 | 1658.7946 (+1), 829.9006 (+2) | ||
| 409–412 | LYSK | 10.2 | 510.2923 (+1) | ||
| 420–426 | WQQGNVF | 29.4 | 878.4145 (+1), 439.7112 (+2) | ||
| 420–439 | WQQGNVFSCSVMHEALHNHY | 37.0 | 1222.5369 (+2), 815.3601 (+3) | ||
| 427–442 | SCSVMHEALHNHYTQK | 21.1 | 1941.8709 (+1), 971.4370 (+2), 647.9609 (+3) | ||
| Light | 29–34 | VGYMHW | 30.9 | 792.3498 (+1), 396.6786 (+2) | |
| 29–35 | VGYMHWY | 33.5 | 955.4126 (+1), 478.2100 (+2) | ||
| 35–41 | YQQKPGK | 9.2 | 848.4616 (+1), 424.7343 (+2) | ||
| 35–44 | YQQKPGKAPK | 10.1 | 1144.6483 (+1), 572.8273 (+2) | ||
| 61–70 | FSGSGSGTEF | 22.3 | 975.4041 (+1) | ||
| 61–85 | FSGSGSGTEFTLTISSLQPDDFATY | 53.3 | 1314.6021 (+2), 876.7387 (+3) | ||
| 61–86 | FSGSGSGTEFTLTISSLQPDDFATYY | 55.2 | 1396.1324 (+2), 931.0921 (+3) | ||
| 61–95 | FSGSGSGTEFTLTISSLQPDDFATYYCFQGSGYPF | 61.4 | 1917.8441 (+2), 1278.8977 (+3) | ||
| 86–102 | YCFQGSGYPFTFGGGTK | 39.1 | 1873.8233 (+1), 937.4132 (+2) | ||
| 87–102 | CFQGSGYPFTFGGGTK | 35.7 | 1710.7601 (+1), 855.8825 (+2), 570.9245 (+3) | ||
| 96–102 | TFGGGTK | 11.6 | 667.3405 (+1), 334.1739 (+2) | ||
| 108–115 | TVAAPSVF | 32.0 | 791.4288 (+1), 396.2183 (+2) | ||
| 116–125 | IFPPSDEQLK | 27.0 | 1173.6152 (+1), 587.3108 (+2) | ||
| 149–172 | VDNALQSGNSQESVTEQDSKDSTY | 21.3 | 1301.5702 (+2), 868.0503 (+3) | ||
| 149–178 | VDNALQSGNSQESVTEQDSKDSTYSLSSTL | 32.1 | 1595.7279 (+2), 1064.1545 (+3) | ||
| 169–172 | DSTY | 9.7 | 485.1877 (+1) | ||
| 169–178 | DSTYSLSSTL | 30.5 | 1073.4993 (+1), 537.2533 (+2) | ||
| 173–182 | SLSSTLTLSK | 28.0 | 1036.5891 (+1), 518.7982 (+2) | ||
| 175–182 | SSTLTLSK | 28.0 | 836.4731 (+1) | ||
| 192–206 | ACEVTHQGLSSPVTK | 20.9 | 1613.7978 (+1), 807.4016 (+2), 538.6037 (+3) |
RT = retention time.