Skip to main content
. 2020 Jul 16;16(7):e1008484. doi: 10.1371/journal.pgen.1008484

Fig 3. Amino acid substitution, Glc7-Q48K, confers 2DG resistance.

Fig 3

A. 2DG resistance assay in glc7Δ cells containing plasmids to express HA-tagged wildtype Glc7 or HA-tagged Glc7-Q48K. B. Western blot of Snf1 kinase using antibodies to detect total Snf1 protein, Snf1 protein phosphorylated on threonine 210 (Snf1-P), or HA-tagged Glc7 protein. A plot of the ratio of phospho-Snf1 to total Snf1 is shown below for triplicate extracts grown prepared before and after incubation in 0.1% 2DG for 2 hours. A representative western blot is shown above. C. Invertase activity of three independent cultures grown in high glucose (H) or two hours after shifting to low glucose (L). Individual data points are shown with the mean indicated with a solid bar. Values statistically different from wild-type cells in the same glucose condition are indicated. D. Western blot of Mig1-3HA protein in cells grown in high (H) glucose or two hours after shifting to 0,05% glucose (L). Relevant genotypes are shown above. Migration of Mig1 and phosphorylated Mig1 are indicated.