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. 2020 Jul 16;16(7):e1008484. doi: 10.1371/journal.pgen.1008484

Fig 4. Amino acid substitution, Snf1-G53R, confers 2DG resistance.

Fig 4

A. 2DG resistance assay in snf1Δ cells transformed with plasmids expressing either HA-tagged wild-type Snf1, HA-tagged Snf1-G53R, or no Snf1 (snf1Δ). B. Western blot of HA-tagged Snf1 protein kinase using antibodies that detect total Snf1 protein or Snf1 protein phosphorylated on threonine 210. Cells were harvested in mid-log phase or two hours after addition of 2DG to 0.1% as indicated. Quantitation of the western signals is shown in the bar graphs above the blot images. C. Invertase activity was measured in three independent cultures grown in high glucose (H) or two hours after shifting to 0.05% glucose (L). Values statistically different from wild-type values in the same glucose condition are indicated.