Table 3. Activity of HXK2 and variants.
HXK2 Allele |
2DG Resistance % Growth in 0.1% 2DG |
Catalytic Activity nmole/min/A.U. |
Invertase Activity mU/OD |
---|---|---|---|
WT HXK2 | 20 | 4.317 | 53 |
hxk2-WRF | 16 | 3.077 | 58 |
hxk2-R423T | 61 | 0.127 | 45 |
hxk2-D417G | 62 | 0.558 | 60 |
hxk2-WCA | 73 | 0.452 | 134 |
hxk2-G55V | 81 | 0.017 | 416 |
hxk2Δ | 80 | 0.0 | 611 |
hxk2-D211A | 81 | 0.0 | 1020 |