Fig. 1.

Down-regulatory Efficiency of recombinant shRNA plasmids on TGF-β1 of the treated cells. The shTGFβ1-1~4 groups were transfected with the recombinant plasmid. The pS-PRRSV group was transfected with pNeg instead of recombinant interfering plasmid, while the DMRIE-C group was treated with the transfection reagent only. After incubation for 36 h, the mRNA level of TGF-β1 is determined. All experiments are performed in triplicate. All results were shown as means±SD. In all cases, different superscript letters indicate that there are statistically significant differences among groups (P<0.05), and vice versa (P>0.05). The followings are the same as here.