Table 2.
Primers for Real-Time qPCR.
| Gene | GeneBank Accession Number | Sequence(5′−3′) | Annealing Temperature ( °C) | Product Length (bp) |
|---|---|---|---|---|
| PRRSV | — —1 | F2:TATCGTGCCGTTCTATCTT | 61.0 | 205 |
| R3:GAAATGGCTGGTGGTGAG | ||||
| TBP | DQ178129 | F:TACCCACCAACAGTTCAGTAGTTAT | 60.6 | 158 |
| R:TACCCACCAACAGTTCAGTAGTTAT | ||||
| Top2β | AF222921.1 | F: AACTGGATGATGCTAATGATGCT | 51 | 464 |
| R: TGGAAAAACTCCGTATCTGTCTC | ||||
| IL-10 | NM_214,041 | F: ATGGGCGACTTGTTGCTGAC | 60.6 | 215 |
| R: CAGGGCAGAAATTGATGACAG | ||||
| TGF-β1 | NM_214,015 | F: TCCAAGGACCCTTCTCGGAT | 60.0 | 110 |
| R: AAAAACCGAGATGGGCGAGA | ||||
| TGF-β3 | NM_214,198 | F: CCAGCCCGATGAGCACATA | 61.6 | 114 |
| R: AGCCATTCACGCACAGTGTC | ||||
| IL-2 | NM_213,861.1 | F: AGCTCTGGAGGGAGTGCTAA | 62.0 | 116 |
| R: TGTTTCAGATCCCTTTAGTTCCA | ||||
| IL-4 | NM_214,123 | F: GCTGCCCCAGAGAACACGAC | 61.0 | 119 |
| R: AGGTTCCTGTCAAGTCCGCTC | ||||
| IL-8 | NM_213,867 | F: TTCACAAGTCTTCTTGCCTCAAC | 61.0 | 86 |
| R: AAGGTAGGATGGGGGCTGAA | ||||
| TNF-α | NM_214,022 | F: CCCACGTTGTAGCCAATGTC | 61.5 | 131 |
| R: GAGGTACAGCCCATCTGTCG | ||||
| IFN-γ | NM_213,948 | F:GAAGAATTGGAAAGAGGAGAGTGAC | 61.5 | 114 |
| R: TCCATGCTCCTTTGAATGGC | ||||
| IFN-α | TQ839262.1 | F: ACTTCCACAGACTCACCCTCTATC | 54.5 | 79 |
| R: ATGACTTCTGCCCTGATGATCT | ||||
| IL-27p28 | NM_001007520 | F: CAGGAGTTGCAGAGGGAGTTCAAG | 61.3 | 102 |
| R: TGGCAGGTGTGATTCAGCAAAGT | ||||
| TLR3 | NM_001097444 | F: GCATTGCCTGGTTTGTTAGTTG | 56.0 | 122 |
| R: TGTATCAAAAAGAATCACTGGGAG | ||||
| TLR7 | NM_001097434.1 | F: TTTCCTCAAATGCCTAAACTTAT | 54.0 | 185 |
| R: AGTGGCTGTTACTACTTATATCT | ||||
| MyD88 | NM_001099923..1 | F: ATCTGCTACTGGCCCAGCGATAC | 61.6 | 101 |
| R: ACATCTCGGTCAGACACACATAACT |
1, the primer of PRRSV is designed based on conserved region of several different virus isolations.
2: forward primer;.
3: reverse primer;.