Table 2.
Sequences (5´ to 3´) of primers used for RT-qPCR and resulting amplicon sizes.
| Gene | Forward primer | Reverse primer | Amplicon Size [bp] |
|---|---|---|---|
| gadX | ATGACGCCCACAGAGTATCAG | GGTCAGTGCCGTAAAAATTCCC | 101 |
| gadA/B | TTACCAGGTTGCCGCTTATC | ACGCAGACGTTCAGAGAGGT | 163 |
| eae | ACCGTCATATCCGGCATTAG | ACCATGACGGTAATCGATCC | 169 |
| tir | TCAGGGGAGAAAACAATGAACG | GCCACTACCTTCACAAACAGAC | 101 |
| cesT | CGACAGATAACCCTAACTTCGC | CCATCGACTTAACGACGACTTC | 186 |
| escU | GGGCGATGTAACAAAAAGTGA | CCGCACCTAATATTTCACGA | 176 |
| cysG | TTTCTGGGAGAAATTGTTCG | TTGCAGTCCTTTCAGTGTCA | 181 |
| gapdh | TCCGTGCTGCTCAGAAACG | CACTTTCTTCGCACCAGCG | 299 |