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. Author manuscript; available in PMC: 2020 Jul 28.
Published in final edited form as: Mol Reprod Dev. 2019 Sep 23;86(12):1822–1831. doi: 10.1002/mrd.23276

FIGURE 1.

FIGURE 1

Nuclear envelope conformation and chromatin configuration in cat germinal vesicles after desiccation and vitrification. (A) Representative micrographs of different categories of the nuclear envelope (NE) conformation (immunostaining of lamin A/C; a–h) and chromatin configurations (DAPI staining; a’–h’). Scale bar = 10 μm. (B) Proportion of different categories of nuclear envelope conformation and chromatin configuration in different treatment groups. Proportions with different letters (spherical NE/reticular chromatin category: capital letters; irregular NE/abnormal chromatin category: lowercase letters) differ within the category (p < .05). Numbers on the top of the bars indicate a total number of oocytes in each treatment group. (C) Areas of nuclear envelope relative to the area of the in oocytes after different treatments. The dotted line represents 1:1 ratio in which the size of chromatin equals that of the nuclear envelope