Skip to main content
. Author manuscript; available in PMC: 2020 Jul 28.
Published in final edited form as: Mol Reprod Dev. 2019 Sep 23;86(12):1822–1831. doi: 10.1002/mrd.23276

FIGURE 2.

FIGURE 2

Relative fluorescent intensity of epigenetic markers in cat GVs following desiccation or vitrification. (A) Representatives of GVs immunostained with H3K4me3 (a, b) or H3K9me3 (c, d) antibodies. Corresponding DNA was counterstained with DAPI (a’–d’). Scale bar = 20 μm. (B, C) Influence of desiccation and vitrification on the intensity of (B) H3K4me3 and (C) H3K9me3. Mean fluorescent intensity of control, untreated GVs was set as 1. Values are mean ± SEM. Values with different letters differ (p < .05). Numbers at the bottom of the bars indicate total number of oocytes in each group. GV, germinal vesicle; SEM, standard error of the mean