Table 1.
Differential phenotypic characteristics of strain CL23T and Robertkochia marina CC-AMO-30DT
Strains: 1, CL23T; 2, R. marina CC-AMO-30DT. All data were obtained from this study. +, Positive reaction; –, negative reaction; w, weakly positive reaction. All strains were positive for: catalase; hydrolysis of xylan and aesculin; production of acid from ᴅ-glucose, aesculin ferric citrate, cellobiose, maltose, sucrose, trehalose, melezitose, starch and glycogen in API 50 CHB strips; and activity of alkali phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, chymotrypsin, acid phosphatase, naphtol-AS-BI-phosphohydrolase, α-glucosidase, β-glucosidase and N-acetyl-β-glucosaminidase. Both strains were negative for flexirubin-type pigment; growth under anaerobic condition; growth on R2A, NA, LBA, TSA and MHA media; hydrolysis of casein, starch, CMC, Tween 80, xanthine and hypoxanthine; nitrate reduction; indole and H2S production; urease; acid production from glycerol, erythritol, ᴅ-arabinose, ʟ-arabinose, ᴅ-ribose, ᴅ-xylose, ʟ-xylose, ᴅ-adonitol, methyl β-ᴅ-xylopyranoside, ᴅ-fructose, ʟ-sorbose, ʟ-rhamnose, dulcitol, inositol, ᴅ-mannitol, ᴅ-sorbitol, methyl α-ᴅ-mannopyranoside, N-acetyl-glucosamine, amygdalin, inulin, xylitol, ᴅ-lyxose, ᴅ-tagatose, ᴅ-fucose, ʟ-fucose, ᴅ-arabitol, ʟ-arabitol, potassium gluconate, potassium 2-ketogluconate and potassium 5-ketogluconate in API 50 CHB strips; and activity of lipase (C14) and β-glucuronidase (API ZYM).
|
Characteristics |
1 |
2 |
|---|---|---|
|
Colony pigmentation |
Yellow |
Orange |
|
Oxidase activity |
+ |
– |
|
Growth parameters: |
|
|
|
pH range |
5–9 |
6–7 |
|
Temperature range (°C) |
15–42 |
20–40 |
|
Temperature optimum (°C) |
30–37 |
30 |
|
NaCl range (%, w/v) |
0–9 |
0.5–4 |
|
NaCl optimum (%, w/v) |
1–2 |
2 |
|
Hydrolysis of: |
|
|
|
Tween 20 |
+ |
w |
|
Tween 40 |
+ |
– |
|
Tween 60 |
+ |
w |
|
Tyrosine |
w |
– |
|
Gelatin |
– |
+ |
|
Production of acetoin |
+ |
– |
|
Oxidation of amygdalin |
w |
– |
|
Utilization of: |
|
|
|
ᴅ-Galactose |
+ |
– |
|
ᴅ-Mannose |
+ |
– |
|
Arbutin |
w |
– |
|
Salicin |
w |
– |
|
Lactose |
+ |
– |
|
Melibiose |
+ |
– |
|
Raffinose |
+ |
– |
|
Gentiobiose |
+ |
– |
|
ᴅ-Turanose |
w |
– |
|
Enzyme activity (API ZYM): |
|
|
|
α-Galactosidase |
+ |
– |
|
β-Galactosidase |
+ |
– |
|
α-Mannosidase |
+ |
w |
|
α-Fucosidase |
w |
– |
|
Antibiotic susceptibility (per disc): |
|
|
|
Ampicillin (10 µg) |
– |
+ |
|
Penicillin G (10 IU) |
– |
+ |
|
Piperacillin (100 µg) |
– |
+ |
|
Bacitracin (45 µg) |
– |
+ |