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. Author manuscript; available in PMC: 2020 Jul 28.
Published in final edited form as: J Immunol. 2016 Oct 28;197(11):4413–4424. doi: 10.4049/jimmunol.1600282

Figure 4. Inhibition of intracellular signaling molecules confirms their role in synergistic induction of TNF and IL-1β by newborn MoDCs.

Figure 4

Newborn and adult MoDCs were pre-treated with Benzimidazole (IRAK1/4 inhibitor; 5 and 50 μM) (A,B), R406 (Syk inhibitor; 5 and 50 μM) (C,D), Celastrol (NF-κB inhibitor; 1 and 10 μM) (E,F) or VX-765 (Caspase-1 inhibitor; 10 or 100 μM) (G,H) for 1 hour prior to activation with R848, TDB, or R848+TDB. Secretion of TNF and IL-1β was measured in supernatant after an 18-hour stimulation. Cytokine production was normalized to condition with no pharmacological inhibitors to visualize the percentage of inhibition. (n=6, mean+SEM, paired Student’s t-test, *p<0.05, **p<0.01 and ***p<0.001)