Skip to main content
. 2020 Jul 28;9:e56177. doi: 10.7554/eLife.56177

Figure 2. Ovariectomy protects female mice lacking hepatic Rictor during midlife.

(A) Kaplan-Meier plot of the survival of female mice in which cancer was observed during gross necropsy (N = 37 female mice; WT Sham 11, L-RKO Sham 9, WT OVX 10, L-RKO OVX 7; Supplementary file 1). (B) Kaplan-Meier plot of the survival of female mice in which cancer was not observed during gross necropsy (N = 36 female mice; WT Sham 11, L-RKO Sham 8, WT OVX 7, L-RKO OVX 10; Supplementary file 1). (C) Kaplan-Meier plot of the survival of female mice lacking hepatic Rictor (L-RKO) and their wild-type (WT) littermates. All mice were subjected to gonadectomy (OVX) or Sham surgery at 3 weeks of age (N = 115 female mice; WT Sham 31, L-RKO Sham 29, WT OVX 27, L-RKO OVX 28; Supplementary file 1). (A–C) The overall effect of genotype (RKO), gonadectomy (OVX), and the interaction was determined using a Cox-proportional hazards test (HR, hazard ratio). The two-sided log-rank sum p-value was then calculated comparing individual curves for effects identified as significant in the regression analysis, and corrected for multiple comparisons (Holm-Sidak).

Figure 2.

Figure 2—figure supplement 1. Frequency of cancer observed at necropsy in female mice.

Figure 2—figure supplement 1.

Frequency at which cancer was observed (bottom) or not observed (top) in female mice. N = 73 female mice; p-value was determined by chi-square analysis.