Figure 5. Independent effects of hepatic Rictor deletion and gonadectomy on glucose tolerance and insulin resistance.

(A–D) The glucose tolerance of (A, B) male and (C, D) female mice lacking hepatic Rictor (L-RKO) and their wild-type (WT) littermates, of the indicated ages and surgical treatments, was determined following an overnight fast. Area under the curve: the overall effect of genotype (RKO), gonadectomy (OVX or CAST), and the interaction represent the p-value from a two-way ANOVA; *=p < 0.05 from a Holm-Sidak post-test examining the effect of parameters identified as significant in the two-way ANOVA (n = Males: A) 9–12 mice/group, 3–5 months of age; B) 7–12 mice/group, 10–12 months of age. Females: C) 7–11 mice/group, 3–5 months of age; D) 7–11 mice/group, 10–12 months of age). (E–F) fasting blood glucose and insulin values were used to calculate HOMA2-IR in E) male and F) female 14 month old mice (n = 4–5 mice/group; the overall effect of genotype (RKO), gonadectomy (OVX or CAST), and the interaction represent the p-value from a two-way ANOVA; *=p < 0.05 from a Holm-Sidak post-test examining the effect of parameters identified as significant in the two-way ANOVA). Error bars represent SEM.

Figure 5—figure supplement 1. Effect of hepatic Rictor loss and gonadectomy on insulin tolerance.

(A–D) The insulin tolerance of (A, B) male and (C, D) female mice lacking hepatic Rictor (L-RKO) and their wild-type (WT) littermates, of the indicated ages and surgical treatments was determined following an overnight fast. Area under the curve: the overall effect of genotype (RKO), gonadectomy (OVX or CAST), and the interaction represent the p-value from a two-way ANOVA; *=p < 0.05 from a Holm-Sidak post-test examining the effect of parameters identified as significant in the two-way ANOVA. (n = Male: A) 9–12 mice/group, 4–5 months of age; (B) 8–9 mice/group, 12–14 months of age. Females: (C) 8–11 mice/group, 3.5–6 months of age; (D) 7–10 mice/group, 12–14 months of age). Error bars represent SEM.

Figure 5—figure supplement 2. Effect of hepatic Rictor loss and gonadectomy on glucose stimulated insulin secretion.

Glucose stimulated insulin levels were measured 15 min after I.P. administration of 1 g/kg glucose (n = 4–5 mice/group; the overall effect of genotype (RKO), gonadectomy (OVX or CAST), and the interaction represent the p-value from a two-way ANOVA). Error bars represent SEM.