Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers |
Additional information |
|---|---|---|---|---|
| Biological Sample | Hyclone fetal bovine serum (FBS) | GE Healthcare Life Sciences | SV30180.03 | |
| Antibody | Monoclonal mouse IgG1 kappa anti human DNAM-1 (CD226) (clone 11A8); FITC | BioLegend | 338304 | 5 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human NKG2D (CD314) (clone 1D11); PE | eBioscience | 12-5878-42 | 5 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human CD56 (clone N901); ECD (PE-Texas Red) | Beckman Coulter | A82943 | 2.5 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human CD16 (clone 3G8); PerCP-Cy5.5 | BioLegend | 302028 | 5 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human NKp46 (CD335) (clone 9E2); PE-Cy7 | BioLegend | 331916 | 5 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human NKp30 (CD337) (clone P30-15); Alexa Fluor 647 | BioLegend | 325212 | 5 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human CD3 (clone UCHT1); Alexa Fluor 700 | BioLegend | 300424 | 2 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human CD19 (clone HIB19); Alexa Fluor 700 | BioLegend | 302226 | 1 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human CD8 (clone SK1); APC-Cy7 | BioLegend | 344714 | 2.5 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG2b anti human CD85j (ILT2) (clone GHI/75); FITC | Miltenyi Biotec | 130-098-437 | 10 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human LAIR-1 (CD305) (clone DX26); PE | BD Biosciences | 550811 | 20 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG2b anti human NKG2A (CD159a) (clone Z199); PE-Cy7(PC7) | Beckman Coulter | B10246 | 20 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human NKp44 (CD336) (clone P44-8); Alexa Fluor 647 | BioLegend | 325112 | 5 μl per tube / 106cells |
| Antibody | Monoclonal mouse IgG1 kappa anti human 2B4 (CD244.2) (clone C1.7); FITC | BioLegend | 329506 | 5 μl per tube / 106cells |
| Chemical Compound | LIVE/DEAD Fixable Violet Dead Stain | Thermo Fisher Scientific | L34955 | 1 μl in 1 μl |
| Chemical Compound | Novagen Benzonase Nuclease | Merck Millipore | 70664 | |
| Chemical Compound | CTL Wash Solution | Cellular Technology Limited | CTLW-010 | |
| Chemical Compound | Trypan Blue viability stain | Santa Cruz | sc-216028 | |
| Chemical Compound | Dimethyl sulfoxide (DMSO) | Santa Cruz | sc-202581 | |
| Chemical Compound | Calbiochem bovine serum albumin (BSA) | Merck Millipore | 2905-OP | |
| Chemical Compound | Sigma-Aldrich sodium azide | Merck Millipore | S8032 | |
| Chemical Compound | Sigma-Aldrich lithium heparin | Merck Millipore | H0878 | |
| Chemical Compound | Ficoll-Paque | GE Healthcare Life Sciences | 17-1440-03 | |
| Chemical Compound | Isoton II isotonic buffered saline solution | Beckman Coulter | 844 80 11 | |
| Chemical Compound | RPMI medium | Lonza | 12-167Q | |
| Chemical Compound | Phosphate Buffered Saline (PBS) | Lonza | 17-517Q | |
| Other | Leucosep tubes | Greiner Bio-One International | 227290 | |
| Software | Kaluza v1.3 | Beckman Coulter |