Table 11. Antibody panels for measuring the phenotype of Natural Killer cells.
| Antibody | Fluorochrome | Clone no. | Supplier |
|---|---|---|---|
| Panel 1 | |||
| DNAM-1 (CD226) | FITC | 11A8 | BioLegend |
| NKG2D (CD314) | PE | 1D11 | eBioscience |
| CD56 | ECD (PE-Texas Red) | N901 | Beckman Coulter |
| CD16 | PerCP-Cy5.5 | 3G8 | BioLegend |
| NKp46 (CD335) | PE-Cy7 | 9E2 | BioLegend |
| NKp30 (CD337) | Alexa Fluor 647 | P30-15 | BioLegend |
| CD3 | Alexa Fluor 700 | UCHT1 | BioLegend |
| CD19 | Alexa Fluor 700 | HIB19 | BioLegend |
| CD8 | APC-Cy7 | SK1 | BioLegend |
| Live/Dead | Dye (violet) | Thermo Fisher Scientific | |
| Panel 2 | |||
| CD85j (ILT2) | FITC | GHI/75 | Miltenyi Biotec |
| LAIR-1 (CD305) | PE | DX26 | BD Biosciences |
| CD56 | ECD (PE-Texas Red) | N901 | Beckman Coulter |
| CD16 | PerCP-Cy5.5 | 3G8 | BioLegend |
| NKG2A (CD159a) | PC7 (PE-Cy7) | Z199 | Beckman Coulter |
| NKp44 (CD336) | Alexa Fluor 647 | P44-8 | BioLegend |
| CD3 | Alexa Fluor 700 | UCHT1 | BioLegend |
| CD19 | Alexa Fluor 700 | HIB19 | BioLegend |
| CD8 | APC-Cy7 | SK1 | BioLegend |
| LIVE/DEAD | Dye (violet) | Thermo Fisher Scientific | |
| Panel 3 | |||
| 2B4 (CD244.2) | FITC | C1.7 | BioLegend |
| CD56 | ECD (PE-Texas Red) | N901 | Beckman Coulter |
| CD16 | PerCp-Cy5.5 | 3G8 | BioLegend |
| CD3 | Alexa Fluor 700 | UCHT1 | BioLegend |
| CD19 | Alexa Fluor 700 | HIB19 | BioLegend |
| CD8 | APC-Cy7 | SK1 | BioLegend |
| LIVE/DEAD | Dye (violet) | Thermo Fisher Scientific | |