Skip to main content
. 2020 Jul 3;46(3):1135–1145. doi: 10.3892/ijmm.2020.4667

Figure 1.

Figure 1

Propofol inhibits HCC cell proliferation, migration and invasion, and induces cell apoptosis in vitro, as well as represses tumor growth in vivo. Cell proliferation in (A) Hep3B and (B) Huh-7 cells untreated or treated with different concentrations of propofol (2.5, 5 or 10 µg/ml) was measured using a MTT assay. (C) Cell apoptosis in Hep3B and Huh-7 cells treated with 5 µg/ml propofol was analyzed via flow cytometry analysis. (D) Protein expression levels of Bcl-2 and c-caspase 3 in Hep3B and Huh-7 cells treated with 5 µg/ml propofol were examined using western blotting. (E) Migration was (F) determined, along with (G) invasion in Hep3B and Huh-7 cells treated with 5 µg/ml propofol using a Transwell assay. Scale bar, 100 µm. (H) Tumor volume was detected every 4 days after Hep3B cells were injected into the mice. (I) Tumor weight was measured after the mice were euthanized. Experiments were repeated three times. *P<0.05 vs. Mock. PI, propidium iodide; OD, optical density; c- cleaved.