Skip to main content
. 2020 Jul 3;46(3):1135–1145. doi: 10.3892/ijmm.2020.4667

Figure 4.

Figure 4

HOXA11-AS negatively regulates miR-4458 expression via direct targeting in HCC cells. (A) Putative binding sites between HOXA11-AS and miR-4458 were predicted using starBase 2.0. Luciferase activity in HOXA11-AS WT or HOXA11-AS MUT and miR-4458 or miR-NC co-transfected (B) Hep3B and (C) Huh-7 cells was detected via dual-luciferase reporter assay. (D) RNA pull-down assay was conducted to assess the interaction between HOXA11-AS and miR-4458, and the expression of HOXA11-AS was detected using RT-qPCR after Bio-miR-4458 or Bio-NC was transfected into Hep3B and Huh-7 cells. (E) Expression of HOXA11-AS in Hep3B and Huh-7 cells transfected with si-NC, si-HOXA11-AS#1, si-HOXA11-AS#2 or si-HOXA11-AS#3 was determined via RT-qPCR. (F) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with Vector or HOXA11-AS was determined via RT-qPCR. (G) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with si-NC or si-HOXA11-AS#1 was measured using RT-qPCR. Experiments were repeated three times. *P<0.05. HOXA11-AS, HOMEOBOX A11 antisense RNA; NC, negative control; miR, microRNA; WT, wild-type; MUT, mutant; RT-qPCR, reverse transcription-quantitative PCR; bio, Biotinylated; si, small interfering RNA.