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. 2020 Jul 3;46(3):1135–1145. doi: 10.3892/ijmm.2020.4667

Figure 5.

Figure 5

miR-4458 inhibition abolishes the effects of HOXA11-AS knockdown on cell progression in HCC cells. Hep3B and Huh-7 cells were transfected with si-NC, si-HOXA11-AS#1, si-HOXA11-AS#1+anti-miR-NC or si-HOXA11-AS#1+anti-miR-4458. (A) Expression of miR-4458 in Hep3B and Huh-7 cells was measured using reverse transcription-quantitative PCR. Cell proliferation was analyzed by MTT assay in (B) Hep3B and (C) Huh-7 cells. (D) Cell apoptosis was detected via flow cytometry analysis. Protein expression levels of PCNA, cyclinD1 and C-Myc in (E) Hep3B and (F) Huh-7 cells were analyzed using western blotting. Protein expression levels of Bcl-2, Bax, c-caspase3 and c-PRRP in (G) Hep3B and (H) Huh-7 cells were analyzed using western blotting. Cell (I) migration and (J) invasion were assessed using Transwell assay. Protein expression levels of E-cadherin, N-cadherin and Vimentin in (K) Hep3B and (L) Huh-7 cells were analyzed using western blotting. Experiments were repeated three times. *P<0.05. HOXA11-AS, HOMEOBOX A11 antisense RNA; NC, negative control; miR, microRNA; si, small interfering RNA; OD, optical density.