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. 2020 Jun 17;46(3):1063–1073. doi: 10.3892/ijmm.2020.4647

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Influence of SIRT1 silencing on TGFβ1-induced HMC and HK-2 cells. HMC and HK-2 cells were transfected with siRNA against human SIRT1 (siSIRT1), and effect of SIRT1 downregulation in miR-135a-5p-knocked down cell was evaluated using western blotting. (A) Levels of SIRT1 were detected. (B) Expression of collagen1A1, α-SMA, FN and E-cadherin was measured. The quantification was performed on Image J. Data were plotted as mean ± standard error of mean and performed in triplicate. ^*P<0.05 vs. control cells (anti-NC or anti-miR-135a-5p+scramble). FN, fibronectin; SMA, smooth muscle actin; TGF, transforming growth factor; HMC, human mesangial cells; miR, microRNA; E, epithelial; NC, negative control; si, small interfering.

Influence of SIRT1 silencing on TGFβ1-induced HMC and HK-2 cells. HMC and HK-2 cells were transfected with siRNA against human SIRT1 (siSIRT1), and effect of SIRT1 downregulation in miR-135a-5p-knocked down cell was evaluated using western blotting. (A) Levels of SIRT1 were detected. (B) Expression of collagen1A1, α-SMA, FN and E-cadherin was measured. The quantification was performed on Image J. Data were plotted as mean ± standard error of mean and performed in triplicate. ^*P<0.05 vs. control cells (anti-NC or anti-miR-135a-5p+scramble). FN, fibronectin; SMA, smooth muscle actin; TGF, transforming growth factor; HMC, human mesangial cells; miR, microRNA; E, epithelial; NC, negative control; si, small interfering.