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. 2020 Jul 9;46(3):1029–1038. doi: 10.3892/ijmm.2020.4672

Figure 2.

Figure 2

Effects of PM, OM and LINC00707 silencing on osteogenic differentiation of HBMSCs. (A) Alizarin Red Staining of PM and OM were performed on day 21. Scale bar, 200 µm. Magnification, ×200. (B) LINC00707 expression after transfection with blank, siNC or siLINC00707 in HBMSCs cultured in OM was analyzed by reverse transcription-quantitative PCR. Each experiment was repeated three times, GAPDH served as an internal control. (C) Alizarin Red Staining of blank, siNC and siLINC00707 in HBMSCs cultured in OM was performed on day 21. (D) Western blotting results of the (E) protein expression levels of osteogenesis-related genes (ALP, OCN, OPN and RUNX2) in blank, siNC and siLINC00707 group. Each experiment was repeated three times, GAPDH served as an internal control. *P<0.05, **P<0.01, ***P<0.001 vs. siNC. HBMSCs, human bone mesenchymal stem cells; PM, proliferation medium; OM, osteogenic medium; NC, negative control; LINC00707, Long intergenic non-protein coding RNA 00707; ALP, Alkaline phosphatase; OCN, osteocalcin; OPN, osteopontin; RUNX2, RUNX family transcription factor 2; siRNA, small interfering RNA.