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. 2020 Jul 9;46(3):1029–1038. doi: 10.3892/ijmm.2020.4672

Figure 4.

Figure 4

Effects of miR-103a-3p and LINC00707 on expression levels of osteogenesis-related genes of HBMSCs. (A) Expression of miR-103a-3p after trans-fection with MC, M, IC, I or siLINC00707 + I in HBMSCs was analyzed by RT-qPCR. Each experiment was repeated three times, and U6 served as an internal control. (B) Western blotting results of the (C) protein expression levels of osteogenesis-related genes (ALP, OCN, OPN and RUNX2) in MC, M, IC, I and siLINC00707 + I HBMSCs. (D) mRNA expression levels of osteogenesis-related genes (ALP, OCN, OPN and RUNX2) in MC, M, IC, I and siLINC00707 + I HBMSCs were detected by RT-qPCR. Each experiment was repeated three times, and GAPDH served as an internal control. *P<0.05, **P<0.01, ***P<0.001 vs. MC; ###P<0.001 vs. IC; ^^^P<0.001 vs. I. HBMSCs, human bone mesenchymal stem cells; RT-qPCR, reverse transcription-quantitative PCR; MC, mimic control; IC, inhibitor control; I, inhibitor; M, mimic; LINC00707, Long intergenic non-protein coding RNA 00707; siRNA, small interfering RNA; ALP, Alkaline phosphatase; OCN, osteocalcin; OPN, osteopontin; RUNX2, RUNX family transcription factor 2.