Figure 2.

CORMs reduce blue light-induced cytotoxicity in RPE cells. (A) RPE cells were preincubated with or without 25 µM CORM2 and CORM3 for 3 h and stimulated with blue light for 24 h. Cell lysates were detected by western blotting with anti-PARP-1. (B) Mitochondrial membrane potential was evaluated using JC-1 dye. Red fluorescence indicates healthy mitochondria and green fluorescence indicates mitochondria in injured cells with low membrane potential. Scale bar, 50 µm. (C) The fluorescent images of mitochondrial membrane potential of RPE cells were determined by the fluorescent dye JC-1 under a fluorescence microscope and expressed as red/green fluorescence intensity ratios. ^*P<0.05 compared with the untreated cells, ^#P<0.05 compared with blue light alone. RPE, retinal pigment epithelium; CORM, carbon monoxide-releasing molecule; PARP-1, poly(ADP-ribose) polymerase 1; c-PARP-1, cleaved PARP-1.