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. 2020 Jul 6;46(3):1155–1165. doi: 10.3892/ijmm.2020.4670

Figure 2.

Figure 2

MEF2A is a target gene regulated by miR-144-3p. (A) mRNA expression of MEF2A in MM primary cells and cell lines was evaluated by RT-qPCR. **P<0.01 vs. normal donor. (B) Luciferase reporter vectors encoding wild-type (WT) or mutant (MUT) MEF2A-3′UTR were co-transfected into 293T cells with miR-144-3p mimics or NC control to determine the relative luciferase activity. ***P<0.001 vs. negative control. (C and D) RT-qPCR and western blot analysis were used to detect MEF2A mRNA and protein expression in RPMI-8226 and U266 cells transfected with miR-144-3p mimics. **P<0.01 vs. negative control (E and F) pcDNA-MEF2A vector and miR-144-3p mimic were used to co-transfect RPMI-8226 and U266 cells. RT-qPCR and western blot analysis were used to detect the mRNA and protein recovery of MEF2A. **P<0.01, ***P<0.001 vs. miR-144-3p mimic. MM, multiple myeloma.