Figure 3.

Knockdown of MEF2A inhibits the proliferation and migration of MM cells. (A) mRNA and (B) protein expression levels of MEF2A were determined following transfection of RPMI-8226 and U266 cells with siRNA1, which exhibited optimal knockdown effects. ^**P<0.01, ^***P<0.001 vs. negative control. (C) Proliferation of RPMI8226 and U266 cells transfected with si-MEF2A was detected using CTG assays. ^*P<0.05, ^**P<0.01 vs. negative control. (D) Transwell assays were used to analyse the migration capacities of RPMI-8226 and U266 cells transfected with different vectors and siRNAs. ^**P<0.01 vs. negative control. (E and F) RPMI-8226 and U266 cells were co-transfected with miR-144-3p mimic and pcDNA-MEF2A, and cell proliferation and migration were evaluated. ^*P<0.05, ^**P<0.01 vs. miR-144-3p mimic. Scale bar, 10 µm. MM, multiple myeloma.