(A-E) tSNE plots of all T cells (A) with
expression levels of CD4 (B), CD8 (C), NK1.1
(D), and PD-L1 (E) from all samples with number-
and color-coded clusters.
(F) Heat map of normalized marker expression of T-cell
clusters with the fractions of four gates. Gate source shows the pooled samples
in each group at the bottom line of the heat map. Black: control, blue:
anti-OX40 monotherapy, magenta: anti–PD-1 monotherapy, and orange:
combination of anti–PD-1 and anti-OX40. Tm: memory T cells, Tex: PD-1
exhausted T cells, and DN: CD4 and CD8 double negative T cells.
(G-I) tSNE plots of all samples and control,
anti-OX40, anti–PD-1, and anti–PD-1 and anti-OX40 groups.
(G) Density plots. Arrow in the anti-OX40 plot indicates
GATA-3 CD4 cells.
(H) Plots of OX40 expression.
(I) Plots of PD-1 expression.