Figure 4. Recognition of exon 8A 5′ss by U1 snRNA.

A diagram of exons 6 through 8 and intervening is given. Exons are shown as colored boxes, introns as lines/broken lines. Splicing events are indicated by dashed arrows. The sequence of exon 8 flanking the 8A 5′ss is given above the exon diagram. Numbering is given relative to the 8A 5′ss. The location of the 5′ss is indicated by an arrow. U1 snRNA is shown above. Base pairs between the 5′ portion of U1 snRNA and the 5′ss are indicated by dots; black dots indicate canonical base pairs and red dots indicate wobble base pairs. The primary splicing product of exon 8A recognition, C6–7–8A, is shown to the right. Of note, other circRNAs including additional exons are also produced downstream of recognition of the 5′ss of exon 8A (See Figures 2 and 3).