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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Gastroenterology. 2020 Jun 24;159(1):289–305.e16. doi: 10.1053/j.gastro.2020.03.013

Figure 3. ABL1 Knockdown reduces human HCC cell proliferation and suppresses tumor growth.

Figure 3.

(A) ABL1 and GAPDH protein expression in scrambled-RNA and ABL1-KD Hep3B and Huh7 cells was determined by Western blotting. (B) Quantification of cell proliferation from scrambled-RNA and ABL1-KD Hep3B and Huh7 cells at different time points after seeding. (C) SCID-bg mice were injected on their flanks with scrambled-RNA (left) and ABL1-KD Huh7 cells (right); after 40 days, gross images of tumors are shown. (D) Tumor weight from scrambled-RNA and ABL1-KD Huh7 cell-injected mice (n=5). (E) Tumor volumes from scrambled-RNA and ABL1-KD Huh7 cell-injected mice (n=5/group). (F) Ki67 staining of the tumors was examined by IHC. (G) Quantification of Ki67 staining. (H) Apoptosis of the tumors was examined by TUNEL staining. (I) Quantification of TUNEL staining.