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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Gastroenterology. 2020 Jun 24;159(1):289–305.e16. doi: 10.1053/j.gastro.2020.03.013

Figure 5. Knockdown of ABL1 decreases NOTCH1 expression through regulation of c-MYC in HCC cells.

Figure 5.

(A) GSEA shows that the MYC targets are enriched in ABL1-KD Huh7 cells. (B) Levels of c-MYC, p-c-MYC (Ser62) and GAPDH proteins in scrambled-RNA and ABL1-KD HCC cells was determined by Western blotting. (C) The interaction of ABL1 and c-MYC was examined using PLA in Huh7 cells. (D) Relative mRNA levels of NOTCH1 in scrambled-RNA control and c-MYC-KD HCC cells. (E) Expression of c-MYC, NOTCH1 and GAPDH proteins in scrambled-RNA and c-MYC-KD HCC cells was determined by Western blotting. (F) Quantification of cell proliferation from scrambled-RNA and c-MYC-KD HCC cells. (G) Expression of MycER (activated fusion c-MYC), NOTCH1, ABL1 and GAPDH proteins in control (infected with the pBpuro retroviral particle) and c-MYC-overexpressed (infected with the pBpuro c-MYCER™ retroviral particle) Huh7 scrambled and ABL1-KD cells was determined by Western blotting. (H) Quantification of cell proliferation from in control and c-MYC-overexpressed Huh7 scrambled and ABL1-KD cells.