|
Uniform
13C-labeling and
15N- labeling at the indicated residues:
|
1 |
K16, F20, V24, G37 |
2 |
D7, G9, E11.L17, F19, A21 |
3 |
E11, F19, 131, V36 |
4 |
E11.L17.A21, M35, G38 |
5 |
I32, M35, G37, V40 |
6 |
Q15, V18, A21 |
7 |
S8, Y10, V12, L34, G38, 141 |
8 |
V12, E22, S26, N27, G33 |
9 |
V12, F20, D23, K28, G29 |
10 |
E11, H13, Q15, L17 |
11 |
E11, K16, F19, V36 |
12 |
A2, E3, F4, G9, V39 |
13 |
H14, K16, L34, A42 |
14 |
F19, V24, G25, A30, 131, L34, M35 [33] |
|
Selective
13C-labeling at the indicated sites:
|
A |
L17 CO |
B |
V18 CO |
C |
F19 CO |
D |
F20 CO |
E |
A21 CO |
F |
L17 CO and F19 CO |
G |
V18COandA21 CO |
H |
L17 CO and A21 CO |
I |
V18 CO and A21 CO (50% isotope-diluted) |