Table 2.

Mutation candidates to increase the affinity. The mutations with highest capability to increase the binding affinity between the ACE2 and RBD are listed in the table.

Wild-type	Res-number	Mutant	Mcsm-ppi2-prediction (ΔΔGaffinity) Kcal/mol	Affinity
THR	27	ARG	1.371	Increasing
LYS	31	GLU	1.311	Increasing
ASP	38	VAL	1.27	Increasing
THR	27	GLU	1.26	Increasing
PHE	28	TRP	1.152	Increasing