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. 2020 Jul 28;10:12562. doi: 10.1038/s41598-020-69548-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Apoptotic exosome-like vesicles decrease the expression of endothelial markers in endothelial cells. Apoptotic exosome-like vesicles (ApoExo) decreased CD31 and vWF expressions in endothelial cells. (A) CD31 and vWF expression by flow cytometry analysis in serum-starved endothelial cells exposed for 24 h to the vehicle (Ctrl) or apoptotic exosome-like vesicles (ApoExo). Flow cytometry experiments expressed as the percentage of vehicle-treated cells median fluorescence intensity (50,000 events/sample) ± SEM (right). Representative gates of CD31 and vWF expression are depicted (left). n ≥ 5 for each condition. P values obtained by a one-sample t-test. (B) ApoExo decreased CD31 expression in endothelial cells by confocal microscopy. (Scale bar: 20 µm). Semi-quantitative measurement of CD31 expression depicted as corrected total cell fluorescence (CTCF) ± SEM (right). Representative pictures of CD31 expression are presented (left). CTCF represents the integrated density minus the area of selected cell multiplied by the mean fluorescence of background reading. n = 3 for each condition. P value obtained by unpaired t-test.