. 2020 Jul 28;18:36. doi: 10.1186/s43141-020-00053-7

Table 2.

Biochemical properties of cold-adapted proteases (published from 2010 onwards)

Microbial source	Protease type	Strong inhibitors	Stimulators/enhancers	Optimum temp./pH	Reference
Acinetobacter sp.	Serine peptidase	PMSF, EDTA, EGTA, 2-ME, Pepstatin A, DTT, and Hg²⁺	Ca²⁺, Mn²⁺, Na⁺, Zn²⁺	40/9	[63]
Bacillus sp.	Metalloprotease	Cu²⁺, Zn²⁺, Hg²⁺, EDTA, and SDS	Mg²⁺and Ca²⁺	40/7.4	[12]
Bacillus sp.	Metalloprotease	EDTA, EGTA, Ca²⁺, Cu²⁺, Mg²⁺, K⁺, Zn²⁺	Mn²⁺	20/9	[34]
Bacillus subtilis	Serine protease	Mg²⁺, Pb²⁺, Mn²⁺, Al³⁺, and Fe²⁺	Cu²⁺ and Ca²⁺	15/10	[30]
Chryseobacterium sp.	Serine protease	Butanol, acetonitrile, isopropanol, ethyl acetate, tetrahydrofuran, Hg²⁺, Zn²⁺, and Cu²⁺	Na⁺, Ca²⁺, Ba²⁺, and Fe²⁺	10/7.0–8.0	[32]
Geomyces pannorum	Aspartic protease	Methanol, isopropanol, DMSO, Mg²⁺, Fe²⁺, Ca²⁺, and Zn²⁺	Co²⁺, Mn²⁺, Cu²⁺, and Ni²⁺	60/3.0	[15]
Halobacillus sp.	Thermolysin-like protease	EGTA, Cu²⁺, EDTA, 1,10-phenanthroline, and Ni²⁺	Mn²⁺, Ca²⁺, Mg²⁺, and Ba²⁺	30/8	[37]
Lysobacter sp.	Serine peptidase	PMSF, EDTA, and Zn²⁺	Ca²⁺, Mg²⁺, Ba²⁺, Na⁺, NH₄⁺, and isopropyl alcohol	40/9.0	[18]
Penicillium nalgiovense	Serine protease	PMSF, SDS, Mn²⁺, and Zn²⁺	Ca²⁺ and Mg²⁺	35/8	[40]
Planococcus sp.	Serine protease	PMSF, DEPC, EDAC, urea, SDS, EDTA, Co²⁺, Zn²⁺, Fe³⁺, and Ni²⁺	Ca²⁺	35/10	[23]
Planococcus sp.	Serine protease	EDTA, PMSF, TNBS, EDAC, Cu²⁺, and Ni²⁺	Fe³⁺ and Ca²⁺	35/10	[60]
Planomicrobium sp.	Serine protease	PMSF and AEBSF	Ca²⁺ and Mn²⁺	35/9	[11]
Pseudomonas aeruginosa	Serine protease	PMSF and Ag⁺	Mg²⁺, K⁺, Ca²⁺, Ba²⁺, and Zn²⁺	25/10	[21]
Pseudoalteromonas sp.	Serine protease	PMSF, SDS, and H₂O₂,	Nm	25–35/8–9	[16]
Pseudoalteromonas arctica	Subtilisin-like protease	Linear alkylbenzene sulfonate (LAS) and SDS	Ca²⁺	30/9.0	[64]
Pseudomonas lundensis	Metalloprotease	EDTA, EGTA, Cu²⁺, Fe³⁺, Al³⁺, Fe²⁺, Mn²⁺, Al³⁺, and Co²⁺	Na⁺, K⁺, and Li⁺	30/10.4	[19]
Serratia marcescens	Metalloprotease	EDTA, MnCl₂, CaCl₂, CoSo₄, HgCl₂, and Na₂	Nm	40/8	[27]
Sporobolomyces roseus	Aspartic protease	2-Mercaptoethanol, dithiothreitol, SDS, and Pepstatin A	Nm	50/4	[31]
Stenotrophomonas sp.	Alkaline protease	Zn²⁺, Cu²⁺, and Co²⁺	Mg²⁺, Mn²⁺, and Ca²⁺	15/10	[28]
Stenotrophomonas maltophilia	Alkaline protease	Co²⁺	Cu²⁺, Cr²⁺	20/10	[29]

Nm not mentioned