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List of FITC-conjugated antibodies for isolation and purification of CD8+ T-cells from lymph nodes of donor OT-I x GFP mice using the negative selection magnetic sorting method.
| Antibodies | Clone | Removal of non-target cells |
|---|---|---|
| CD4 | H129.19 | CD4 T-cells |
| CD19 | MB19-1 | B-cells |
| CD49b | DX5 | NK cells |
| MHCII i[Ab] | AF6-120.1 | Antigen-presenting cells |
| CD25 | PC61 | Activated T-cells |
| CD69 | H1.2F3 | Activated T-cells |
