FIGURE 3.

Taxonomic composition and relative abundances of organisms represented by metagenome assembled genomes (MAGs) from WWTP samples over a period of three years. Relative proportions between MAGs were determined based on average contig coverages of each MAG in relation to the overall coverage of binned contigs for each metagenome, thereby correcting for potential differences in genome size and completeness. The fraction of sequence information that could be successfully binned represented 67–73% of the sequence data that could be assembled into contigs > 1 kbp (see also Supplementary Figure 7). The resulting relative abundances between binned taxa largely reflect the corresponding taxon proportions of the community observed via marker gene analyses of the complete metagenomes (see also Figure 2).