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. 2020 Jul 22;11:1377. doi: 10.3389/fmicb.2020.01377

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Copyright © 2020 Dam, Vollmers, Sobol, Cabezas and Kaster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis: (A) 16S rRNA genes of Chloroflexi single amplified genomes (SAGs) constructed using the Arb software. Partial 16S rRNA genes of approximately 500 bp were amplified from SAGs; their sequences were aligned using the online SINA platform and imported into Arb. 16S rRNA gene sequences extracted from genomes of Chloroflexi isolates are included as references. (B) Distance-based phylogeny on the basis of gene content of Chloroflexi genomes obtained via SCG and metagenomics approaches. Classification at the class level is given on the right. Streptomyces griseus is used as an outgroup. Nodes with bootstrap values of more than 50 are presented in the tree. Scale bars indicate a 10% sequence divergence.