Figure 3.

OVX mice manifest serious sepsis consequences with a weaker trained immunity effect than female mice. (A) Serum E₂ concentration was significantly decreased in OVX mice. (B) The lung was visualized by H&E staining, microscopically analyzed, and histologically scored by a pathologist. (C) Kidney E. coli burden in OVX mice compared with female mice at indicated time points following model in Figure 1A. (D,E) Level of serum ALT and AST were detected in OVX and female mice treated with E. coli or TI + E. coli groups. (F,G) Serum IL-6 and TNFα were measured by ELISA to characterize the different extent of the trained immunity response between OVX and female mice. (H) PBMCs from OVX mice trended toward M2 polarization in E. coli group compared to female mice (n = 5/group). *p < 0.05, **p < 0.01, and ***p < 0.001 as determined by Student's t-test.