Figure 4.

Female BMDMs are apt to be polarized to M1 phenotype in responses to trained immunity in vitro. (A) Female BMDMs M1 polarization was stronger than male BMDMs treated with LPS or TI + LPS groups. (B) Very few female BMDMs to polarized to M2 in LPS group, while male BMDMs showed the polarization of M2 phenotype. TI suppressed BMDMs to M2 polarization from both male and female mice (n ≥ 3/group). ***p < 0.001, paired Student's t-test comparing LPS group and TI + LPS group in the same gender. ^#p < 0.05, ^###p < 0.001, paired Student's t-test comparing same group between different genders.