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. 2020 Jul 22;11:1591. doi: 10.3389/fimmu.2020.01591

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Copyright © 2020 Sun, Pan, Qu, Xu, Dou and Hou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of E₂ on nuclear translocation of RelB contributes to macrophage polarization to change the intensity of trained immunity. (A) Immunofluorescence indicated that E₂ treatment inhibited nucleus translocation of RelB but not P65 in the HEK293T cell line. (B) Immunofluorescence indicated that E₂ treatment inhibited nuclear translocation of RelB but not P65 in BMDMs. (C) The knockdown function of ERα siRNA was verified by testing the ERα mRNA level in HEK293T and RAW264.7. (D) The knockdown function of ERα siRNA was verified by testing ERα protein expression level in RAW264.7. (E) Nuclear protein was extracted to determine the effect of E₂ on the content of RelB in nuclear in RAW264.7. (F) The effect of E₂ on the downstream gene of RelB, IL-4, and IL-10, in RAW264.7 was confirmed by PCR (n ≥ 3/group). *p < 0.05, **p < 0.01, and ***p < 0.001, paired Student's t-test comparing with control group.