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. 2020 Jul 22;8:853. doi: 10.3389/fbioe.2020.00853

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Copyright © 2020 Vallejo, Peltier, Garrigós, Matallana, Marullo and Aranda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gln3 is required for respiration and glucose repression. (A) The spot analysis of mutants gln3Δ in three genetic backgrounds, BQS252, C9, and Σ1278b in medium with ammonium as the nitrogen source (SD) and proline as the nitrogen source (SPro). (B) The spot analysis of the same mutants in YPSucrose with or without 100 mg/ml of 2-deoxyglucose (2DG) to test glucose repression. (C) The same strains in YPGlycerol to check for respiration. (D) The zymogram of alcohol dehydrogenase activity. Samples of the gln3Δ mutants in BY4742, C9, and Σ1278b genetic backgrounds were taken under the exponential (repressing) and postdiauxic (derepressing) conditions, and run in a non-denaturing PAGE gel. Alcohol dehydrogenase isoforms I and II of strain Σ1278b are indicated by arrows.