Table 3.

Minimum inhibitory concentrations (MIC) were calculated for fresh Bald’s eyesalve (n = 12; batches 19–30), garlic only preparations (n = 3) and external allicin standards (n = 3).

Treatment	Allicin concentration range (mean ± s.e.)	Allicin concentration in MIC	Biofilm activity (log drop from control)	Average predicted allicin concentration in biofilm treatment
ESO	275—486 (391 ± 19) μg·ml-1	11—39 μg·ml-1	Eradicated biofilm (> 5)	142 μg·ml-1
Garlic only	358—434 (389 ± 19) μg·ml-1	45—54 μg·ml-1	No activity (0)	160 μg·ml-1
Allicin Standard		62.5 μg·ml-1	Partial activity (2.6)	167 μg·ml-1

Allicin concentrations for each batch were quantified using HPLC and calculated using an allicin calibration curve. MICs were measured in synthetic wound fluid. The allicin concentration in the MIC of each batch, and the allicin concentration present in the biofilm treatment experiments, is calculated and the results of biofilm treatment experiments are provided for reference. Raw data is supplied in the Data Supplement.