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. 2020 Jul 28;10:12644. doi: 10.1038/s41598-020-69267-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mechanical stretching modulates AKT phosphorylation through β1 integrin and ILK. (a) Immunoblot of human tendon cells that were bound to RGDS-conjugated magnetic particles and exposed to one hour of oscillatory magnetic field (MF) with a frequency of 1 Hz. “- “ and “ + ” indicates without or with magnetic field, respectively. (b) shows the densitometry of phospho-AKT normalized to Vinculin in (a). (c) Immunoblot of human tendon cells incubated with anti-integrin β1 conjugated magnetic particles after 1 h of oscillatory magnetic field (MF) with a frequency of 1 Hz. The immunoblots of human tendon cells incubated with ILK inhibitor QLT0267. (d) shows the densitometry of phospho-AKT normalized to Vinculin in (c). (e), and siILK (g) indicate preventing AKT phosphorylation by mechanical stretching (ST) with Flexcell compared to the non-stretched (NS) cells. (f), (g) show the densitometry of phospho-AKT normalized to Vinculin in (e) and (g), respectively. Two-way ANOVA followed by Bonferroni's multiple comparisons test; mean ± SE; ns P > 0.05; * P ≤ 0.05; **P ≤ 0.01; n ≥ 4 biological replicates.