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. 2020 Jul 28;10:12644. doi: 10.1038/s41598-020-69267-6

Figure 4.

Figure 4

Global changes of protein synthesis and eIF4F complex formation on cap after mechanical stimulation. (a) Representative images of SUnSET/Western blot analysis of new protein synthesis in tendon-derived stromal and C3H10T1/2 cells after mechanical stretching. (b) shows the densitometry analysis of anti-puromycin blots normalized to vinculin. (c) Lysates from non-stimulated and stimulated (mechanical stretching) human tendon cells were subjected to m7GTP pull-downs and analyzed for the indicated proteins by Western blot. Ratio paired t-test; mean ± SE; ns P > 0.05; * P ≤ 0.05; ***P ≤ 0.001; n ≥ 3 biological replicates.