Table 3.
Molecular detection of Anaplasma spp. and Ehrlichia spp. in biological samples (blood or spleen) of Xenarthra mammals.
| Xenarthra species (nº sampled) | PCR assays for Anaplasma spp.—nº positive (%)/nº positive by state | PCR assays for Ehrlichia spp.—nºpositive (%)/nº positive by state | ||
|---|---|---|---|---|
| nPCR screening (rrs gene) | cPCR (23S–5S intergenic region) | qPCR (msp-2 gene) | cPCR screening (dsb gene) | |
| Bradypus variegatus (188) | 65 (34.57%)/34 PA, 31 RO | 39 (20.74%)/18 PA, 19 RO | 2 (3.07%)/2 PA | 56 (29.78%)/42PA, 14RO |
| Bradypus sp. (3) | 2 (66.66%)/2 RO | 2 (66.6%)/2 RO | 0 | 2 (66.66%)/2 RO |
| Choloepus didactylus (5) | 2 (40%)/2 PA | 1 (20%)/1 PA | 0 | 1 (20%)/1PA |
| Choloepus sp (31) | 13 (41.93%)/13 RO | 6 (19.35%)/6 RO | 0 | 5 (16.12%)/5 RO |
| Tamandua tetradactyla (31) | 5 (16.12%)/1 PA, 4 SP | 3 (9.677%)/3 SP | 5 (16.12%)/1PA, 4 SP | 10 (32.25%)/1 PA, 4 MS, 5 SP |
| Myrmecophaga tridactyla (52) | 0 | NT | NT | 7 (13.46%)/3 SP, 4 MS |
| Cabassous unicinctus (3) | 2 (66.66%)/2 MS | 0 | 0 | 0 |
| Dasypus novemcinctus (8) | 1 (12.5%)/1 MS | 0 | 0 | 0 |
| Euphractus sexcinctus (8) | 1 (12.5%)/1 MS | 0 | 0 | 0 |
| Priodontes maximus (1) | 0 | NT | NT | 0 |
| Total = 330 | 91 | 51 | 7 | 81 |
PA Para, RO Rondônia, SP São Paulo, MS Mato Grosso do Sul, NT samples not tested due to negative results in the screening PCR assays.