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. 2020 Jul 28;10:12639. doi: 10.1038/s41598-020-68876-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Slices from an intact rabbit brain embedded by paraffin, stained with H&E. (A) The coronal plane slices from the rabbit brain. (B) Magnification of (A). (C) Magnification of (B). Blood vessels indicated by the black arrow. (D) Magnification of (A). (E, F) Magnification of (D). (G) The coronal plane slices from the rabbit brain. All the sections were sectioned at the coronal plane for 4 μm, and we collected serial 50 images. (H) 3D reconstructed images of (G). (I) Magnification of (H). The meninx indicated by the white arrow. (J) Magnification of (I). (K, L) Magnification of (H). Scale bars, (A) 500 μm; (B, C) 50 μm; (D) 100 μm; (E) 50 μm; (F) 25 μm; (G) 500 μm; (H) white grid, 1 mm; (I) white grid, 100 μm; (J) white grid, 50 μm; (K, L) white grid, 100 μm.