Table 3.
Paraffin processing schedules.
| Regent | Mouse brain (0.4 cm3) |
Rat brain (1.6 cm3) |
Rabbit brain (10 cm3) |
Macaque hemisphere (60 cm3) |
|---|---|---|---|---|
| 4% paraformaldehyde (PFA) | 12 h | 24 h | 3 × 24 h | 4 × 48 h |
| 0.01 M phosphate buffer (PBS) | 12 h | 24 h | 3 × 24 h | 5 × 24 h |
| 50% (vol/vol) alcohol | 3 × 1 h | 2 × 3 h | 3 × 4 h | 2 × 12 h |
| 70% (vol/vol) alcohol | 3 × 1 h | 2 × 3 h | 3 × 4 h | 2 × 12 h |
| 80% (vol/vol) alcohol | 3 × 1 h | 2 × 3 h | 3 × 4 h | 2 × 12 h |
| 95% (vol/vol) alcohol | 2 × 1 h | 2 × 3 h | 3 × 4 h | 2 × 12 h |
| 100% (vol/vol) alcohol | 3 × 1 h | 3 × 2 h | 3 × 4 h | 3 × 24 h |
| 50% (vol/vol) Histo-clear II | 1 h | 2 × 1 h | 3 × 3 h | 2 × 12 h |
| 100% (vol/vol) Histo-clear II | ≥ 45 min | ≥ 1 h | ≥ 2 h | ≥ 12 h |
| Paraffin-immersion | 3 × 4 h | 3 × 8 h | 4 × 12 h | 8 × 24 h |
| Embedding | 2–3 h | 2–3 h | 6 h | Overnight |