. 2020 Jul 28;10:12575. doi: 10.1038/s41598-020-69424-x

Table 2.

Development of lymph node and lung metastases after coinjection with different fibroblast primary cultures in orthotopic LuCaP136 and LNCaP xenografts. 10 weeks after intraprostatic injection of 5 × 10⁵ LuCaP136 or LNCaP cells combined with 5 × 10⁵ CAF1, NCAF1 or BPHF1 cells, mice were sacrificed, and their organs were examined for the presence of metastases. The number of animals with lymph node and lung metastases in each of the 6 resulting groups is given in this table (based on histological examination of the removed organs). The results from the first series, in which LuCaP136 and LNCaP cells were injected alone (see also Table 1), are added as well. Single animals in different combination groups (initially n = 8 in both groups) died prematurely due to not cancer-specific causes. p values were determined by Fisher´s exact test. BPHF = benign prostate hyperplasia associated fibroblasts, CAF = cancer-associated fibroblasts, NCAF = non-cancer-associated fibroblasts, LN = lymph node.

	≥ 2 LN metastases	Statistical significance	Lung metastases	Statistical significance
LuCaP136 alone (n = 6)	2/6	/	0/6	/
LuCaP136 + NCAF1 (n = 6)	1/6	p = 1,00 (vs. LuCaP136 alone)	0/6	p = 1,00 (vs. LuCaP136 alone)
LuCaP136 + BPHF1 (n = 7)	2/7	p = 1,00 (vs. LuCaP136 alone) p = 0,63 (vs. LuCaP136 + NCAF1)	1/7	p = 1,00 (vs. LuCaP136 alone) p = 1,00 (vs. LuCaP136 + NCAF1)
LuCaP136 + CAF1 (n = 7)	6/7	p = 0,10 (vs. LuCaP136 alone) p = 0,03 (vs. LuCaP136 + NCAF1) p = 0,10 (vs. LuCaP136 + BPHF1)	4/7	p = 0,07 (vs. LuCaP136 alone) p = 0,07 (vs. LuCaP136 + NCAF1) p = 0,27 (vs. LuCaP136 + BPHF1)
LNCaP alone (n = 7)	1/7	/	1/7	/
LNCaP + NCAF1 (n = 7)	1/7	p = 1,00 (vs. LNCaP alone)	2/7	p = 1,00 (vs. LNCaP alone)
LNCaP + BPHF1 (n = 7)	2/7	p = 1,00 (vs. LNCaP alone) p = 1,00 (vs. LNCaP + NCAF1)	2/7	p = 1,00 (vs. LNCaP alone) p = 1,00 (vs. LNCaP + NCAF1)
LNCaP + CAF1 (n = 7)	4/7	p = 0,27 (vs. LNCaP alone) p = 0,27 (vs. LNCaP + NCAF1) p = 0,59 (vs. LNCaP + BPHF1)	5/7	p = 0,10 (vs. LNCaP alone) p = 0,29 (vs. LNCaP + NCAF1) p = 0,29 (vs. LNCaP + BPHF1)