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. 2020 Jul 28;10:12618. doi: 10.1038/s41598-020-69313-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The dense interaction network of L7/L12 captured by XL-MS. Ribosomal proteins (black) and rRNA (grey) are shown in cartoon. Cross-linked proteins are color highlighted. Cross-links are shown as dotted lines, satisfaction (yellow) or minor violation (orange) are indicated. (A) Interaction network of L7/L12. Sizes of the boxes are scaled to the length of the protein sequences; each dotted line represents one identified novel cross-link pair. (B) Satisfied cross-links after remodeling; Only 4 cross-links of L6 to L7/L12 could not be unambiguously mapped (not shown); In addition, 5 intra-links show violation in the monomer, but satisfaction in the L7/L12 multimer. (C) Model of the interaction of an L7/L12 tetramer (blue, green, red, orange) bound to the helix 8 of L10 (magenta) based on the cross-linking information (see text). (D) Satisfaction of intra-molecular cross-links between CTD domains of L7/L12. (E) Inter-molecular cross-links satisfying both extended and helical conformation of the linker region of L7/L12; Note that all cross-links are satisfied in the helical linker conformation, and all but one cross-link are satisfied in the extended conformation. The images of protein structures were generated with PyMOL (Version 2.3.2), Schrödinger, LCC. https://pymol.org.