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. 2020 Jul 28;10:12630. doi: 10.1038/s41598-020-69417-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Size and shape of fluorescently-stained conidia in the wild type (WT), Δpks15 and the complemented isolate G6. (a) Calcofluor- and FITC-tagged concanavalin A staining (upper and lower panels). Bars, 5 μm. (b) Distribution of sizes in the conidial populations of the wild type, Δpks15 and G6 from a single representative experiment. (c) Frequency of conidial sizes for each of the three strains. Size data were from three independent experiments. (d) Distribution of shapes in the conidial populations of wild type, Δpks15 and G6 from a single representative experiment. Shape factor (circularity) was determined using the NIS-Elements D software. A shape factor of 1.0 indicates a circle, whereas a shape factor less than 1.0 indicates an ellipse. (e) Frequency of conidial shapes for each of the three strains. Data shown are mean ± s.e.m. Asterisks indicate statistical significance between the wild type or the complemented isolate G6 and Δpks15 (Student’s t-test: *p < 0.05; **< 0.01).