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. 2020 Jul 28;10:12630. doi: 10.1038/s41598-020-69417-w

Table 1.

Mutations in the bar locus of three mutants. The mutants A26 and C1 were directly mutagenized by CRISPR/Cas9.

DNA sequence Insertion\deletion Amino acid sequence
bar-targeting sgRNA
∆pks15 (with a functional bar cassette) 5′..ACCCACCTGCTGAAGTCCCTGGAGGCACAG..3′ ..THLLKSLEAQ.
A26 bar mutant 5′..ACCCACCTGCTGAAGTCCCT TGGAGGCACAG..3′ + 1 ..THLLKSLGGT.
C1 bar mutant 5′..ACCCACCTGCTGAAGTCCCC TGGAGGCACAG..3′ + 1 ..THLLKSPGGT.

The one-base insertions in A26 and C1, highlighted in italics, caused frame shifts and changes in the amino acid sequence. The PAM site is underlined.